Windows ISAPI_Rewrite Not Working

[eluser]Total Shop UK[/eluser]
1) http://www.mysite.com/newsite/index.php?about
2) http://www.mysite.com/newsite/about

Number 1 works but the rewrite number 2 does not?

I have tried with both RewriteBase / and /newsite/

Code:

RewriteBase /
RewriteCond %{REQUEST_FILENAME} !-f
RewriteCond %{REQUEST_FILENAME} !-d
RewriteRule ^(.*)$ index.php/$1 [L]

I've tried all URI Protocols but still no luck.

Thanks